Gel Based Services :

Instrumentation: Biorad SDS-PAGE Unit; Biorad Protean IEF Cell; Image Master 2D Platinum-7 Software (GE)

Requisition Form:

2D RF : Download

• Native PAGE

• Iso-Electric Focussing

• Two-Dimensional Gel Electrophoresis

• Image Analysis by Image Master 2D Platinum-7

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Mass Spectrometry Services :

Instrumentation: Bruker Daltonics Ultraflex III MALDI TOF/TOF; Waters Xevo-G2 XS QTOF; Waters Triple Quadrupole

Requisition Form:

MALDI RF Download
LCMS RF : Download
GCMS : Download

1. MALDI

• MALDI-MW: Molecular weight determination

• MALDI-PMF: Protein Identification (Peptide Mass Fingerprinting)

• MALDI-MSMS: Protein Identification and MSMS fragmentation of peptides

2. LCMSMS

• MW: Molecular weight determination

• Protein Profiling: Protein Identification

• Label-Free Quantification: Protein Identification and Differential expression analysis

• Protein Sequencing

• Post-Translational Modification Studies

• Quantification of free and bound Amino acids

• Quantification Studies using MRM

• Bio-informatic analysis of MS data

3. GCMS

• Carbohydrate analysis

• Fatty acid analysis

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HPLC Services

Instrumentation: Agilent HPLC

Requisition Form:

HPLC RF : Download

• Purity check

• Reverse Phase chromatography

• Size Exclusion chromatography

• Strong Cation Exchange chromatography

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Spectroscopy Services

Instrumentation: CD: Jasco-1500 Spectropolarimeter; Fluorescence: Varioskan Flash

Requisition Form:

CD RF : Download
FLUORESCENCE RF: Download

• Circular Dichroism Spectroscopy: Near UV and Far UV Spectra; Secondary Structure Estimation

• Fluorescence Studies: Absorption and Emission Spectra